| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Bacillus thuringiensis | GCCGAAAGCACTAGGTGGT | TGTGAAACGCCAGCGACA | 60.00 | 60.20 | 225 | 92 |
91.67%
|
61.11%
|
| Primer 12 | Bacillus thuringiensis | TGGCCAACATGCGAGTGT | CATTCGCAACACTTCCGGAC | 59.89 | 59.83 | 209 | 91 |
100.00%
|
22.22%
|
| Primer 13 | Bacillus thuringiensis | TGGCCAAGTGACTCGTGAG | AGCCCAAACCCGATAGCAC | 59.63 | 60.08 | 228 | 91 |
100.00%
|
27.78%
|
| Primer 14 | Bacillus thuringiensis | AGCGAATGGGCATGCAAC | GCGCTGTAACTTCCTGAAACG | 59.11 | 60.14 | 212 | 89 |
100.00%
|
16.67%
|
| Primer 15 | Bacillus thuringiensis | AGAACTCGTGTGGTGGCA | GCAACCGCAAGTAACGCA | 58.77 | 59.37 | 272 | 88 |
100.00%
|
16.67%
|
| Primer 16 | Bacillus thuringiensis | GGGGTAAAAGCTGAGGGAACA | CGCTTTCATTCCACCAGCG | 59.93 | 59.86 | 216 | 88 |
100.00%
|
22.22%
|
| Primer 17 | Bacillus thuringiensis | AGGGGTAAAAGCTGAGGGAAC | CGCTTTCATTCCACCAGCG | 59.65 | 59.86 | 217 | 88 |
100.00%
|
22.22%
|
| Primer 18 | Bacillus thuringiensis | AGGGGTAAAAGCTGAGGGAA | CGCTTTCATTCCACCAGCG | 58.24 | 59.86 | 217 | 88 |
100.00%
|
22.22%
|
| Primer 19 | Bacillus thuringiensis | GGGGTAAAAGCTGAGGGAAC | CGCTTTCATTCCACCAGCG | 58.16 | 59.86 | 216 | 88 |
100.00%
|
22.22%
|
| Primer 20 | Bacillus thuringiensis | TGCGATTGGAAGCGGACT | TCAACCGCAGCCTTCACA | 59.34 | 59.49 | 180 | 87 |
100.00%
|
22.22%
|